Astro Boy: Omega Factor features elements from Astro Boy's different incarnations. The concept of robot rights, Dr. Tenma's ultimate plan for Astro and the robot city of Antarctica are lifted from the Astro Boy 2003 TV series. Plotlines from other Tezuka series include a time travel plot lifted from Marine Express, a journey to the lost continent of Mu, and a subplot involving Duke Red's daughter and her role in the "Death Mask" orbital platform.
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